DNA taken from the body of murdered British tourist Hannah Witheridge matches that of two Asian men, Thai police say.
Officers also said they do not know if the killer is still on the island of Koh Tao, where she and David Miller, 24, were found murdered.
Sky News has obtained video of Ms Witheridge filmed in the hours before she was killed.
In the exclusive footage, she is seen on CCTV walking between bars with a group of friends.
Ms Witheridge, 23, from Great Yarmouth, suffered severe head wounds and Mr Miller died from blows to the head and drowning, post-mortem examinations showed.
Their bodies were found in a rocky area of Sairee beach on Monday.
A garden hoe with Ms Witheridge's blood on it was discovered nearby, and investigators are searching for a blunt metal object used on Mr Miller.
Sky's Jonathan Samuels, on Koh Tao (Turtle Island), said: "Today the head of Thailand's police flew to the island by helicopter - really a sense of how seriously now the Thai authorities are taking this investigation.
"He confirmed that two men's DNA that was found on Hannah's body have been analysed and he has confirmed those two samples are from two Asian men.
"Of course they have no idea at the moment, as far as we understand, who these two men may be.
"They have questioned many people on the island, but so far they tell us they have no firm suspects."
Ms Witheridge is seen leaving a bar with friends DNA on a cigarette butt has been matched to semen found on Ms Witheridge, and officers say the cigarette was smoked by more than one person.
Police Colonel Kissana said officers have still not come up with the exact number of suspects, and confirmed they are looking for both male and female suspects.
He said reports that the FBI are involved in the investigation were incorrect, but confirmed they have British assistance.
A cash reward worth about £4,000 is being offered for information that leads to the arrest of the killers, he added.
Meanwhile, two British brothers who were questioned by police have been told they are free to return home to Jersey.
Christopher and James Ware, childhood friends of Mr Miller, were spoken to by officers but were never detained or named as suspects.
A group of Burmese migrants who were interviewed by police after bloodstains were found on their clothes have also been eliminated from police inquiries.
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